肺炎系列 (二百五十) 深喉唾液 vs 鼻咽和咽喉拭子

 

有好多人問，怎樣化驗冠狀病毒，而作出診斷？

這個問題深入深出，不是三言兩語可以講完，讀吓書先。

  

DTS vs NP

深喉唾液 =  deep-throat saliva (DTS)

鼻咽和咽喉拭子 = Nasopharyngeal specimens (NPsp), e.g. nasopharyngeal swabs (NPS) and aspirates (NPA)

Coronavirus Testing Basics. FDA US food and drug administration.

To KKW et al. Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study. Lancet infectious disease Volume 20, Issue 5, May 2020, Pages 565-574

E. Pasomsub et al. Saliva sample as a non-invasive specimen for the diagnosis of coronavirus disease 2019: a cross-sectional study. Clinical microbiology and infection. May 15, 2020

To KKW et al. Consistent detection of 2019 novel coronavirus in saliva. Clin Infect Dis 2020 Jul 28;71(15):841-843. doi: 10.1093/cid/ciaa149.

To KKW et al. Additional molecular testing of saliva specimens improves the detection of respiratory viruses. Emerg Microbes Infect 2017; 6: e49

Chan JF et al. Improved molecular diagnosis of COVID-19 by the novel, highly sensitive and specific COVID-19-RdRp/Hel real-time reverse transcription-polymerase chain reaction assay validated in vitro and with clinical specimens. J Clin Microbiol 2020

Anne L. Wyllie et al. Saliva is more sensitive for SARS-CoV-2 detection in COVID-19 patients than nasopharyngeal swabs

Sally Cheuk Ying WONG et al.  Posterior oropharyngeal saliva for the detection of SARS-CoV-2. Clinical Infectious Diseases 21 June 2020

Eddie Chi man Leung et al. Deep throat saliva as an alternative diagnostic specimen type for the detection of SARS‐CoV‐2. Journal of Medical Virology 04 July 2020

Christopher K C Lai et al. Prospective study comparing deep-throat saliva with other respiratory tract specimens in the diagnosis of novel coronavirus disease (COVID-19). The Journal of Infectious Diseases 01 August 2020

Abbas Mohammadi et al. SARS-CoV-2 detection in different respiratory sites: A systematic review and meta-analysis. EBioMedicine July 24, 2020

全民檢測｜檢測中心加名額，袁國勇示範自行採樣正確方法，鼻咽、咽喉拭子、深喉唾液。明報 2020-9-4

全民檢測在即，市民對病毒檢測四大謬誤，專科醫生逐一拆解。明報 2020-8-14

 

   

Basics of sensitivity, specificity, PPV and NPV

Robert Trevethan. Sensitivity, Specificity, and Predictive Values: Foundations, Pliabilities, and Pitfalls in Research and Practice. Front Public Health. 2017; 5: 307.

Sensitivity, Specificity, PPV and NPV. Greeky Medics

   

What is PCR?

Polymerase Chain Reaction (PCR) Fact Sheet. National Human Genome Research Institute

What is PCR? Roche Last Updated: 07.08.2020

Polymerase chain reaction (PCR) Khan Academy

  

知之為知之，不知為不知，是知也。

陳沛然議員上